I test booster sopprimono l' LH dai miei dati...

[Bomb Jack]

Originariamente inviato da jim_a

il problema sta nel enzima dopa decarbosilasi che converte l-dopa in dopamina....

cosi non passa molta l-dopa dall BEE..... si converte in dopamina prima di passare la BEE... e provoca alcuni effeti collaterali come nausea, tachicardia e vasocostrizione per stimolazione dei recetori periferici di noradrenalina...

veramente, THEHUGE scrisse che la mucuna ha una farmacocinetica (forse per la presenza di altre sostanze nell'estratto) particolare, che non ha gran necessità d'inibitore della dopa-decarbossilasi.
...comunque pare che l'ecgc possa avere questa funzione.

necessito maggiori informazioni sull'argomento

[Bomb Jack]

P.S. (THEHUGE, dove sei... :-()

[greatescape]

egcg + mucuna

[D. Ace]

Originariamente inviato da Bomb Jack

P.S. (THEHUGE, dove sei... :-()

credo che sia sia smarcato. Io non c'ero quando lui ha scritto, ma mi pare di aver capito che fituncensored.com stesse diventando www.thehugeanswer.com....
peccato

Originariamente inviato da greatescape

egcg + mucuna

ah bè, io credo di avere egcg che mi scorre in corpo 24 h XD

[lupin III]

In realta' non ci si assuefa cosi' presto alla dopa e comunque non avrebbe nemmeno senso assumerla per lunghi periodi.
Posto questo, continuo a ritenere una sciocchezza le affermazioni del tipo meno prolattina = piu' testosterone.
Sara' anche poi che 'sta minchiata (intendo quella affermazione) mi stava rovinando la vita sessuale...

[Bomb Jack]

Originariamente inviato da lupin III

In realta' non ci si assuefa cosi' presto alla dopa e comunque non avrebbe nemmeno senso assumerla per lunghi periodi.
Posto questo, continuo a ritenere una sciocchezza le affermazioni del tipo meno prolattina = piu' testosterone.
Sara' anche poi che 'sta minchiata (intendo quella affermazione) mi stava rovinando la vita sessuale...

Si, chiaro che una roba del genere vada ciclicizzata.
Inoltre, si parla di possibilità di abbassamento della prolattina, non tout-court, ma ove queasta abbia un aumento/picco, proprio come si diceva nelle discussioni con THEHUGE.
Abbassrla per abbassarla non ha senso...

[jim_a]

Originariamente inviato da Bomb Jack

veramente, THEHUGE scrisse che la mucuna ha una farmacocinetica (forse per la presenza di altre sostanze nell'estratto) particolare, che non ha gran necessità d'inibitore della dopa-decarbossilasi.
...comunque pare che l'ecgc possa avere questa funzione.

necessito maggiori informazioni sull'argomento

non sono thehuge.... ti ha gia risposto great....

Originariamente inviato da greatescape

egcg + mucuna

Green tea polyphenols: novel irreversible inhibitors of dopa decarboxylase.
Bertoldi M, Gonsalvi M, Voltattorni CB.
Source

Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Facoltà di Medicina e Chirurgia, Università degli Studi di Verona, Strada Le Grazie, 8, Verona, 37134, Italy.
Abstract

The green tea gallocatechins, (-)-epigallocatechin-3-O-gallate (EGCG), and (-)-epigallocatechin (EGC) were found to be inhibitors of Dopa decarboxylase (DDC). EGCG and EGC inactivate the enzyme in both a time- and concentration-dependent manner and exhibit saturation of the rate of inactivation at high concentrations, with efficiency of inactivation values (k(inact)/K(i)) of 868 and 1511 M(-1) min(-1), respectively. In contrast, gallic acid behaves as a weak inhibitor of DDC. Protection against inactivation by EGCG and EGC was observed in the presence of the active site-directed inhibitor D-Dopa. Either EGCG or EGC induce changes in the absorbance and CD bands of the visible spectrum of enzyme-bound PLP. Taken together, these findings indicate the active site nature of the interaction of DDC with both polyphenols. On the basis of the properties of the EGCG-inactivated enzyme, it can be suggested that inactivation could be ascribed to a covalent modification of not yet identified residue(s) of the active site of DDC.

Copyright 2001 Academic Press.


egcg inhibisce anhe le COMT... che degrada la dopamina anche altre catecolamine


Enzymology of Methylation of Tea Catechins and Inhibition of Catechol-O-methyltransferase by (−)-Epigallocatechin Gallate

Hong Lu,
Xiaofeng Meng and
Chung S. Yang

+ Author Affiliations

Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, the State University of New Jersey, Piscataway, New Jersey

Dr. Chung S. Yang, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, 164 Frelinghuysen Road, Piscataway, NJ 08854. E-mail: csyang@rci.rutgers.edu

Abstract

(−)-Epigallocatechin gallate (EGCG) and (−)-epigallocatechin (EGC) are the major polyphenolic constituents in green tea. In this study, we characterized the enzymology of cytosolic catechol-O-methyltransferase (COMT)-catalyzed methylation of EGCG and EGC in humans, mice, and rats. At 1 μM, EGCG was readily methylated by liver cytosolic COMT to 4"-O-methyl-EGCG and then to 4′,4"-di-O-methyl-EGCG; EGC was methylated to 4′-O-methyl-EGC. The Km andVmax values for EGC methylation were higher than EGCG; for example, with human liver cytosol, theKm were 4.0 versus 0.16 μM andVmax were 1.28 versus 0.16 nmol/mg/min. Rat liver cytosol had higher COMT activity than that of humans or mice. The small intestine had lower specific activity than the liver in the methylation of EGCG and EGC. Glucuronidation on the B-ring or thed-ring of EGCG greatly inhibited the methylation on the same ring, but glucuronidation on the A-ring of EGCG or EGC did not affect their methylation. Using EGC and 3,4-dihydroxy-l-phenylalanine as substrates, EGCG, 4"-O-methyl-EGCG, and 4′,4"-di-O-methyl-EGCG were all potent inhibitors (IC50 ∼0.2 μM) of COMT. The COMT-inhibiting activity of (−)-EGCG-3′-O-glucuronide was similar to EGCG, but (−)-EGCG-4"-O-glucuronide was less potent. The present work provides basic information on the methylation of EGCG and suggests that EGCG may inhibit COMT-catalyzed methylation of endogenous and exogenous compounds.

[Bomb Jack]

Originariamente inviato da jim_a

non sono thehuge.... ti ha gia risposto great....




Green tea polyphenols: novel irreversible inhibitors of dopa decarboxylase.
Bertoldi M, Gonsalvi M, Voltattorni CB.
Source

Dipartimento di Scienze Neurologiche e della Visione, Sezione di Chimica Biologica, Facoltà di Medicina e Chirurgia, Università degli Studi di Verona, Strada Le Grazie, 8, Verona, 37134, Italy.
Abstract

The green tea gallocatechins, (-)-epigallocatechin-3-O-gallate (EGCG), and (-)-epigallocatechin (EGC) were found to be inhibitors of Dopa decarboxylase (DDC). EGCG and EGC inactivate the enzyme in both a time- and concentration-dependent manner and exhibit saturation of the rate of inactivation at high concentrations, with efficiency of inactivation values (k(inact)/K(i)) of 868 and 1511 M(-1) min(-1), respectively. In contrast, gallic acid behaves as a weak inhibitor of DDC. Protection against inactivation by EGCG and EGC was observed in the presence of the active site-directed inhibitor D-Dopa. Either EGCG or EGC induce changes in the absorbance and CD bands of the visible spectrum of enzyme-bound PLP. Taken together, these findings indicate the active site nature of the interaction of DDC with both polyphenols. On the basis of the properties of the EGCG-inactivated enzyme, it can be suggested that inactivation could be ascribed to a covalent modification of not yet identified residue(s) of the active site of DDC.

Copyright 2001 Academic Press.


egcg inhibisce anhe le COMT... che degrada la dopamina anche altre catecolamine


Enzymology of Methylation of Tea Catechins and Inhibition of Catechol-O-methyltransferase by (−)-Epigallocatechin Gallate

Hong Lu,
Xiaofeng Meng and
Chung S. Yang

+ Author Affiliations

Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, the State University of New Jersey, Piscataway, New Jersey

Dr. Chung S. Yang, Department of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, 164 Frelinghuysen Road, Piscataway, NJ 08854. E-mail: csyang@rci.rutgers.edu

Abstract

(−)-Epigallocatechin gallate (EGCG) and (−)-epigallocatechin (EGC) are the major polyphenolic constituents in green tea. In this study, we characterized the enzymology of cytosolic catechol-O-methyltransferase (COMT)-catalyzed methylation of EGCG and EGC in humans, mice, and rats. At 1 μM, EGCG was readily methylated by liver cytosolic COMT to 4"-O-methyl-EGCG and then to 4′,4"-di-O-methyl-EGCG; EGC was methylated to 4′-O-methyl-EGC. The Km andVmax values for EGC methylation were higher than EGCG; for example, with human liver cytosol, theKm were 4.0 versus 0.16 μM andVmax were 1.28 versus 0.16 nmol/mg/min. Rat liver cytosol had higher COMT activity than that of humans or mice. The small intestine had lower specific activity than the liver in the methylation of EGCG and EGC. Glucuronidation on the B-ring or thed-ring of EGCG greatly inhibited the methylation on the same ring, but glucuronidation on the A-ring of EGCG or EGC did not affect their methylation. Using EGC and 3,4-dihydroxy-l-phenylalanine as substrates, EGCG, 4"-O-methyl-EGCG, and 4′,4"-di-O-methyl-EGCG were all potent inhibitors (IC50 ∼0.2 μM) of COMT. The COMT-inhibiting activity of (−)-EGCG-3′-O-glucuronide was similar to EGCG, but (−)-EGCG-4"-O-glucuronide was less potent. The present work provides basic information on the methylation of EGCG and suggests that EGCG may inhibit COMT-catalyzed methylation of endogenous and exogenous compounds.

No, ma guarda che io sono d'accordo (e grazie per gli studi, anzi)
Riportavo solo quanto detto da THEHUGE, purtroppo non ne possiamo più discutere ormai...

P.S. comunque sapevo che le COMT non degradano solo la dopamina, ma tutte le catecolamine...

[alessa']

ho letto tutte e 18 pagine con molto interesse, una piccola curiosita, ma alla fine quel prodotto ke doveva uscire di THEHUGE si è saputo qualcosa?? ho notato anke ke THEHUGE nn scrive piu come mai??

[LiborioAsahi]

Avrà altro da fare.
Il prodotto è uscito.

[M.Ivanko]

Il Pow?

[LiborioAsahi]

Intendevo il nuovo bioforge!

[novellino]

Originariamente inviato da LiborioAsahi

Intendevo il nuovo bioforge!

E tu non apprezzi , miscredente

[LiborioAsahi]

Originariamente inviato da novellino

E tu non apprezzi , miscredente

Non è che non apprezzo, è che lo userei in un ottica differente.
Tipo ora che c'è il cambio stagione, per le persone di mezza età potrebbe tornare utile



In realtà non so, quella era la mia prima impressione...un mio amico, MOOOLTO influenzabile ha detto che funziona benissimo , ma potrebbe essere che si è galvanizzato a vedere la pubblicità . Cmq lo proverò prima o poi, son curioso.